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Side A ------ Side B
What are the 2 types of radiation used to kill microorganisms? ------ Gamma and Ultraviolet
What is Gamma radiation also known as? ------ Ionizing radiation, it's greatest impact is when it collides with molecules in the cell, creating free radicals which are toxic
What is Ultraviolet radiation also known as? ------ Non-Ionizing radiation, kills by damaging DNA
Give an example of UV radiation ------ formation of thymine or cytosine dimers (bonds between 2 adjacent pyrimidines in the DNA strand), these lead to mutations
What wavelength of UV light is most lethal? ------ 200-900 nm
Why is UV light as a sterilzing method not a good idea? ------ It has poor penetrating ability, will not go through plastic, glass and many other materials. B/C of this, is is mainly used to kill microbes on the surfaces, in air, or in water
What kind of lamps are used in UV light? ------ Germicidal lamps
What will looking directly into UV raise cause? ------ Eye damage and cataracts, wear goggles
What 2 organisms were used in this experiment? ------ Bacillus cerus and Serratia
What were index cards used for in this experiment? ------ While half of the plates were exposed to UV light, the other half was covered with an index card to indicate non-exposure to UV light

Side A ------ Side B
What are the 2 types of radiation used to kill microorganisms? ------ Gamma and Ultraviolet
What is Gamma radiation also known as? ------ Ionizing radiation, it's greatest impact is when it collides with molecules in the cell, creating free radicals which are toxic
What is Ultraviolet radiation also known as? ------ Non-Ionizing radiation, kills by damaging DNA
Give an example of UV radiation ------ formation of thymine or cytosine dimers (bonds between 2 adjacent pyrimidines in the DNA strand), these lead to mutations
What wavelength of UV light is most lethal? ------ 200-900 nm
Why is UV light as a sterilzing method not a good idea? ------ It has poor penetrating ability, will not go through plastic, glass and many other materials. B/C of this, is is mainly used to kill microbes on the surfaces, in air, or in water
What kind of lamps are used in UV light? ------ Germicidal lamps
What will looking directly into UV raise cause? ------ Eye damage and cataracts, wear goggles
What 2 organisms were used in this experiment? ------ Bacillus cerus and Serratia
What were index cards used for in this experiment? ------ While half of the plates were exposed to UV light, the other half was covered with an index card to indicate non-exposure to UV light